A Reagent for Reduction of Disulfide Bonds in Proteins That Reduces Disulfide Bonds Faster Than Does Dithiothreitol
نویسندگان
چکیده
We have synthesized a new reagent-N,N'-dimethyl-N,.,V.bis(mercaptoacetyl)hydrazine(DMH)-for the reduction of disulfide bonds in proieins. DMH reduce.s disulfide bonds ? times faster than does dithiothreitol (DTT) in water at pH ?. DMH reduces mixed disulfides of cvsteine proteases (papain and ficin) especially rapdily i30 ti-.r faster than DTT). DMH (eo = -0.300 V) reduces noncl'clic disulfides completely, although it is less strongly reducing than DTT (60 = -0.356 V).
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